Development of a microwave-accelerated metal-enhanced fluorescence 40 second, <100 cfu/ml point of care assay for the detection of Chlamydia trachomatis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20709634.
- Also identified by DOI 10.1109/TBME.2010.2066275 and PMC identifier 3221307.
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Abstract
An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.
Medical subject headings
- Chlamydia trachomatis
- Microwaves
- Molecular Diagnostic Techniques
- Point-of-Care Systems
- Spectrometry, Fluorescence