Rapid selection of transgenic C. elegans using antibiotic resistance.
basic_science · Level V
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- Record sourced from PubMed, PMID 20729840.
- Also identified by DOI 10.1038/nmeth.1495.
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Abstract
Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
Medical subject headings
- Caenorhabditis elegans
- Drug Resistance
- Selection, Genetic
- Transgenes