An antibiotic selection marker for nematode transgenesis.
basic_science · Level V
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- Record sourced from PubMed, PMID 20729841.
- Also identified by DOI 10.1038/nmeth.1494.
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Abstract
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
Medical subject headings
- Caenorhabditis
- Drug Resistance
- Gentamicins
- Selection, Genetic
- Transgenes