Autoassembly protein arrays for analyzing antibody cross-reactivity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20804215.
- Also identified by DOI 10.1021/nl1026056 and PMC identifier 3136110.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.
Medical subject headings
- Antibodies
- Cross Reactions
- Nanotechnology
- Protein Array Analysis