Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions.
other · Level V
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- Record sourced from PubMed, PMID 20805793.
- Also identified by DOI 10.1038/nmeth0910-668b and PMC identifier 2945879.
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Abstract
We developed a fast method for denoising pyrosequencing for community 16S rRNA analysis. We observe a 2–4 fold reduction in the number of observed OTUs (operational taxonomic units) comparing denoised with non-denoised data. ~50,000 sequences can be denoised on a laptop within an hour, two orders of magnitude faster than published techniques. We demonstrate the effects of denoising on alpha and beta diversity of large 16S rRNA datasets.
Medical subject headings
- Metagenome
- Sequence Analysis, DNA