Overlap and effective size of the human CD8+ T cell receptor repertoire.
basic_science · Level V
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- Record sourced from PubMed, PMID 20811043.
- Also identified by DOI 10.1126/scitranslmed.3001442 and PMC identifier 3212437.
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Abstract
Diversity in T lymphocyte antigen receptors is generated by somatic rearrangement of T cell receptor (TCR) genes and is concentrated within the third complementarity-determining region 3 (CDR3) of each chain of the TCR heterodimer. We sequenced the CDR3 regions from millions of rearranged TCR beta chain genes in naïve and memory CD8(+) T cells of seven adults. The CDR3 sequence repertoire realized in each individual is strongly biased toward specific V(beta)-J(beta) pair utilization, dominated by sequences containing few inserted nucleotides, and drawn from a defined subset comprising less than 0.1% of the estimated 5 x 10(11) possible sequences. Surprisingly, the overlap in the naïve CD8(+) CDR3 sequence repertoires of any two of the individuals is approximately 7000-fold larger than predicted and appears to be independent of the degree of human leukocyte antigen matching.
Medical subject headings
- CD8-Positive T-Lymphocytes
- Complementarity Determining Regions
- Receptors, Antigen, T-Cell