Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20817248.
- Also identified by DOI 10.1016/j.biomaterials.2010.07.088.
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Abstract
We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.
Medical subject headings
- DNA
- Electrolytes
- Serum Albumin, Bovine
- Transfection