Fluidic microstructuring of alginate hydrogels for the single cell niche.

Braschler, Thomas; Valero, Ana; Colella, Ludovica; Pataky, Kristopher; Brugger, Juergen; Renaud, Philippe · Lab Chip · 2010

basic_science · Level V

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Abstract

Controlling alginate gel formation by diffusion of Ca(2+) ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.

Medical subject headings