Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20824255.
- Also identified by DOI 10.1039/c005243d.
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Abstract
This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.
Medical subject headings
- DNA
- Microfluidic Analytical Techniques
- Microscopy, Fluorescence
- Oligonucleotide Array Sequence Analysis
- Thermometers