Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20871617.
- Also identified by DOI 10.1038/nmeth.1508 and PMC identifier 2967732.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We introduce quantitative dynamic footprinting microscopy to resolve neutrophil rolling on P-selectin. We observed that the footprint of a rolling neutrophil was fourfold larger than previously thought, and that P-selectin-PSGL-1 bonds were relaxed at the leading edge of the rolling cell, compressed under the cell center, and stretched at the trailing edge. Each rolling neutrophil formed three to four long tethers that extended up to 16 μm behind the rolling cell.
Medical subject headings
- Leukocyte Rolling
- Membrane Glycoproteins
- Microscopy, Fluorescence
- Neutrophils
- P-Selectin
- Protein Footprinting