Optogenetic control of heart muscle in vitro and in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20881965.
- Also identified by DOI 10.1038/nmeth.1512.
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Abstract
Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.
Medical subject headings
- Myocytes, Cardiac