Preparation of diethylaminoethyl hollow fibres for high-flow liquid chromatography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 2090312.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A novel anion-exchange column for fast-flow liquid chromatography was developed. The column was prepared by immobilizing diethylaminoethyl groups on to the inner walls of regenerated cellulose hollow fibres. A detailed investigation was conducted of the parameters affecting diethylaminoethyl immobilization such as the NaOH concentration, the reaction temperature, the diethylaminoethyl concentration and the reaction span. Based upon the findings, a protocol to optimize the preparation of the diethylaminoethyl hollow fibres was established. The diethylaminoethyl hollow fibres prepared adsorbed 186 +/- 30 mg of albumin/g fibres.
Medical subject headings
- Chromatography, Ion Exchange
- Diethylamines