Chemical synthesis of the mouse mitochondrial genome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20935651.
- Also identified by DOI 10.1038/nmeth.1515.
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Abstract
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.
Medical subject headings
- DNA, Mitochondrial
- Genome, Mitochondrial