Centrifugal microfluidic system for primary amplification and secondary real-time PCR.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20938554.
- Also identified by DOI 10.1039/c0lc00161a.
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Abstract
Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.
Medical subject headings
- Microfluidic Analytical Techniques
- Reverse Transcriptase Polymerase Chain Reaction