Efficient CNS gene delivery by intravenous injection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20953176.
- Also identified by DOI 10.1038/nmeth.1518.
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Abstract
We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.
Medical subject headings
- Cerebral Cortex
- Gene Transfer Techniques
- Simian virus 40
- Spinal Cord
- Transgenes