Dual RMCE for efficient re-engineering of mouse mutant alleles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20953177.
- Also identified by DOI 10.1038/nmeth.1521 and PMC identifier 3576631.
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Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Medical subject headings
- Embryonic Stem Cells
- Genetic Engineering
- Recombinases