Chronic optical access through a polished and reinforced thinned skull.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20966916.
- Also identified by DOI 10.1038/nmeth.1530 and PMC identifier 3204312.
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Abstract
We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.
Medical subject headings
- Skull