Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20980660.
- Also identified by DOI 10.1073/pnas.1009999107 and PMC identifier 2993355.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Medical subject headings
- Cryoelectron Microscopy
- Eukaryotic Cells
- Models, Molecular
- Protein Biosynthesis
- RNA, Ribosomal
- Ribosomes