Acoustic characterization of nanoswitch structures: application to the DNA Holliday Junction.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21038866.
- Also identified by DOI 10.1021/nl103491v.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A novel biophysical approach in combination with an acoustic device is demonstrated as a sensitive, rapid, and label-free technique for characterizing various structures of the DNA Holliday Junction (J1) nanoswitch. We were successful in discriminating the "closed" from the "open" state, as well as confirming that the digestion of the J1 junction resulted in the two, anticipated, rod-shaped, 20 bp long fragments. Furthermore, we propose a possible structure for the ∼10 nm long (DNA58) component participating in the J1 assembly. This work reveals the potential of acoustic devices as a powerful tool for molecular conformation studies.
Medical subject headings
- Acoustics
- DNA, Cruciform
- Nanostructures