Analyzing marginal cases in differential shotgun proteomics.

Carvalho, Paulo C; Fischer, Juliana S G; Perales, Jonas; Yates, John R; Barbosa, Valmir C; Bareinboim, Elias · Bioinformatics · 2011

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Abstract

We present an approach to statistically pinpoint differentially expressed proteins that have quantitation values near the quantitation threshold and are not identified in all replicates (marginal cases). Our method uses a Bayesian strategy to combine parametric statistics with an empirical distribution built from the reproducibility quality of the technical replicates. The software is freely available for academic use at http://pcarvalho.com/patternlab.

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