Three-dimensional cellular ultrastructure resolved by X-ray microscopy.
basic_science · Level V
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- Record sourced from PubMed, PMID 21076419.
- Also identified by DOI 10.1038/nmeth.1533 and PMC identifier 7337972.
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Abstract
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ∼36-nm (Rayleigh) and ∼70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
Medical subject headings
- Adenocarcinoma
- Microscopy
- Organelles