Senescence in cells in aseptic loosening after total hip replacement.

Abstract

Particle-induced osteolysis is a major cause of aseptic loosening after total joint replacement. The purpose of the current study was to evaluate cellular senescence of macrophages and giant cells in patients with aseptic hip loosening by determination of SA-β-Gal (SA-β-galactosidase), a reliable and frequently used indicator of cellular senescence. The level of senescence in capsule and interface membranes was significantly higher in patients with aseptic loosening in comparison to specimens from patients without aseptic loosening. Using Spearman's rank correlation, we found that the expression of SA-β-Gal in giant cells (p=0.002) and macrophages (p=0.050) in the interface membranes correlates significantly with the degree of polyethylene debris. We speculate that the induction of DNA damage by wear particles is responsible for premature senescence. Consequently, we conclude that the form of senescence observed in this study is a "stress-induced senescence".