A robust system for production of minicircle DNA vectors.
basic_science · Level V
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- Record sourced from PubMed, PMID 21102455.
- Also identified by DOI 10.1038/nbt.1708 and PMC identifier 4144359.
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Abstract
Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.
Medical subject headings
- Biotechnology
- Cloning, Molecular
- DNA, Circular
- Genetic Vectors
- Plasmids