Breaking the diffraction barrier: super-resolution imaging of cells.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 21168201.
- Also identified by DOI 10.1016/j.cell.2010.12.002 and PMC identifier 3272504.
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Abstract
Anyone who has used a light microscope has wished that its resolution could be a little better. Now, after centuries of gradual improvements, fluorescence microscopy has made a quantum leap in its resolving power due, in large part, to advancements over the past several years in a new area of research called super-resolution fluorescence microscopy. In this Primer, we explain the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM. Then, we describe recent applications of super-resolution microscopy in cells, which demonstrate how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.
Medical subject headings
- Microscopy, Fluorescence