Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays.
Level V
Where this comes from
- Record sourced from PubMed, PMID 21187950.
- Also identified by DOI 10.1371/journal.pone.0015243 and PMC identifier 3004854.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant sequences. This improvement was attributed to the lower sequence divergence of RASs, which also expanded the sequence space covered by the microarray. Extension of this novel microarray design strategy to viruses, antimicrobial resistance elements or toxins is straightforward.
Medical subject headings
- Escherichia coli Proteins
- Oligonucleotide Array Sequence Analysis