Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Level V
Where this comes from
- Record sourced from PubMed, PMID 21198335.
- Also identified by DOI 10.3109/14653249.2010.544721.
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Abstract
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
Medical subject headings
- Adipose Tissue
- Cell Culture Techniques
- Cell Separation
- Mesenchymal Stem Cells
- Recombinant Proteins
- Trypsin