Targeted amino-terminal acetylation of recombinant proteins in E. coli.
basic_science · Level V
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- Record sourced from PubMed, PMID 21203426.
- Also identified by DOI 10.1371/journal.pone.0015801 and PMC identifier PMC164640.
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Abstract
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co-expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.