Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging.
basic_science · Level V
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- Record sourced from PubMed, PMID 21209918.
- Also identified by DOI 10.1371/journal.pone.0015881 and PMC identifier PMC1765426.
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Abstract
Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1) enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.