Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21217749.
- Also identified by DOI 10.1038/nmeth.1552 and PMC identifier 3076599.
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Abstract
In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.
Medical subject headings
- Calcium
- Microscopy, Fluorescence, Multiphoton