RNAi screening for fat regulatory genes with SRS microscopy.
basic_science · Level V
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- Record sourced from PubMed, PMID 21240281.
- Also identified by DOI 10.1038/nmeth.1556 and PMC identifier 3061290.
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Abstract
Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.
Medical subject headings
- Caenorhabditis elegans
- Fats
- Gene Expression Regulation
- Microscopy
- RNA Interference
- Spectrum Analysis, Raman