High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21249135.
- Also identified by DOI 10.1371/journal.pone.0016148 and PMC identifier 3017553.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.
Medical subject headings
- Electrophoresis, Capillary
- Gene Products, tat
- HIV-1
- Neurons