Click assembly of magnetic nanovectors for gene delivery.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21255832.
- Also identified by DOI 10.1016/j.biomaterials.2010.12.047 and PMC identifier 4124627.
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Abstract
Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe(3)O(4) nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.
Medical subject headings
- Click Chemistry
- Magnetics
- Nanoparticles
- Transfection