Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21258339.
- Also identified by DOI 10.1038/nmeth.1558 and PMC identifier 3086017.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
Medical subject headings
- Fluorescence Recovery After Photobleaching
- Microscopy, Fluorescence
- Software
- Systems Biology