Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
basic_science · Level V
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- Record sourced from PubMed, PMID 21278748.
- Also identified by DOI 10.1038/nm.2294.
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Abstract
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
Medical subject headings
- Creutzfeldt-Jakob Syndrome