High-throughput single-molecule optofluidic analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21297618.
- Also identified by DOI 10.1038/nmeth.1569 and PMC identifier 3075913.
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Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
Medical subject headings
- DNA-Directed RNA Polymerases
- Fluorescence Resonance Energy Transfer
- High-Throughput Screening Assays
- Microfluidic Analytical Techniques
- RNA, Messenger
- Transcription, Genetic