Controlling the contents of microdroplets by exploiting the permeability of PDMS.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21298160.
- Also identified by DOI 10.1039/c0lc00615g.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) through the PDMS membrane of the device.
Medical subject headings
- Dimethylpolysiloxanes
- Microfluidic Analytical Techniques