Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 21304970.
- Also identified by DOI 10.1371/journal.pone.0016741 and PMC identifier 3031629.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.
Medical subject headings
- Ant Venoms
- Fluoroimmunoassay
- Hypersensitivity, Immediate
- Immunoglobulin E
- Immunoglobulin G