Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21319839.
- Also identified by DOI 10.1021/nl1041947 and PMC identifier 4077779.
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Abstract
We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.
Medical subject headings
- Lipoproteins, HDL
- Molecular Mimicry
- Nanoparticles
- Nucleic Acids