Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21325318.
- Also identified by DOI 10.1098/rsif.2010.0747 and PMC identifier 3119885.
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Abstract
The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV-visible absorption spectroscopy. A value of 1.3×10(7) M(-1) was obtained for the antibody-antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.
Medical subject headings
- Immunoglobulin G
- Metal Nanoparticles
- Silver
- Surface Plasmon Resonance