Isolation of bacterial ribosomes with monolith chromatography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21326610.
- Also identified by DOI 10.1371/journal.pone.0016273 and PMC identifier 3033897.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology.
Medical subject headings
- Anion Exchange Resins
- Bacteria
- Ribosomes