Flower: extracting information from pyrosequencing data.
Where this comes from
- Record sourced from PubMed, PMID 21330287.
- Also identified by DOI 10.1093/bioinformatics/btr063 and PMC identifier 3065695.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The SFF file format produced by Roche's 454 sequencing technology is a compact, binary format that contains the flow values that are used for base and quality calling of the reads. Applications, e.g. in metagenomics, often depend on accurate sequence information, and access to flow values is important to estimate the probability of errors. Unfortunately, the programs supplied by Roche for accessing this information are not publicly available. Flower is a program that can extract the information contained in SFF files, and convert it to various textual output formats. Flower is freely available under the General Public License.
Medical subject headings
- Sequence Analysis, DNA
- Software