R453Plus1Toolbox: an R/Bioconductor package for analyzing Roche 454 Sequencing data.

Klein, Hans-Ulrich; Bartenhagen, Christoph; Kohlmann, Alexander; Grossmann, Vera; Ruckert, Christian; Haferlach, Torsten; Dugas, Martin · Bioinformatics · 2011

basic_science · Level V

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Abstract

The R453Plus1Toolbox is an R/Bioconductor package for the analysis of 454 Sequencing data. Projects generated with Roche's data analysis software can be imported into R allowing advanced and customized analyses within the R/Bioconductor environment for sequencing data. Several methods were implemented extending the current functionality of Roche's software. These extensions include methods for quality assurance and annotation of detected variants. Further, a pipeline for the detection of structural variants, e.g. balanced chromosomal translocations, is provided. The R453Plus1Toolbox is implemented in R and available at http://www.bioconductor.org/. A vignette outlining typical workflows is included in the package. h.klein@uni-muenster.de Supplementary data are available at Bioinformatics online.

Medical subject headings