Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.
basic_science · Level V
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- Record sourced from PubMed, PMID 21383139.
- Also identified by DOI 10.1073/pnas.1101503108 and PMC identifier 3064370.
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Abstract
mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.
Medical subject headings
- Escherichia coli
- RNA, Bacterial
- RNA, Messenger
- RNA, Transfer
- Ribosomes