Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21410218.
- Also identified by DOI 10.1021/nl200285r and PMC identifier 3087289.
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Abstract
The intracellular uptake of 30 nm diameter gold nanoparticles (Au-NPs) was studied at the nanoscale in pristine eukaryotic cells. Live COS-7 cells were maintained in a microfluidic chamber and imaged using scanning transmission electron microscopy. A quantitative image analysis showed that Au-NPs bound to the membranes of vesicles, possibly lysosomes, and occupied 67% of the available surface area. The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles.
Medical subject headings
- Cell Membrane
- Gold
- Microscopy, Electron, Scanning Transmission
- Nanostructures