Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21423760.
- Also identified by DOI 10.1371/journal.pone.0017767 and PMC identifier 3056787.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol.
Medical subject headings
- Extracellular Space
- Phospholipase D
- Phytophthora infestans