Clonal interrogation of stem cells.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 21451511.
- Also identified by DOI 10.1038/nmeth.1590.
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Abstract
Individual stem cells are functionally defined by their self-renewal and differentiation potential. Methods for clonal analysis are essential for understanding stem cells, particularly given the increasing evidence for stem-cell heterogeneity. Stem cells reside within complex microenvironments, making single-cell analysis particularly challenging. Furthermore, simultaneous molecular and functional characterization of single stem cells is not trivial. Here we explore clonal assays applied to stem cell biology and their use in understanding the cellular and molecular basis of stem-cell identity.
Medical subject headings
- Single-Cell Analysis
- Stem Cells