A more efficient method to generate integration-free human iPS cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 21460823.
- Also identified by DOI 10.1038/nmeth.1591.
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Abstract
We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
Medical subject headings
- Cell Culture Techniques
- Induced Pluripotent Stem Cells