A genome-scale shRNA resource for transgenic RNAi in Drosophila.
basic_science · Level V
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- Record sourced from PubMed, PMID 21460824.
- Also identified by DOI 10.1038/nmeth.1592 and PMC identifier 3489273.
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Abstract
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.
Medical subject headings
- Drosophila melanogaster
- Genome, Insect
- RNA Interference