LPA5 is abundantly expressed by human mast cells and important for lysophosphatidic acid induced MIP-1β release.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21464938.
- Also identified by DOI 10.1371/journal.pone.0018192 and PMC identifier 3065470.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release.
Medical subject headings
- Chemokine CCL4
- Lysophospholipids
- Mast Cells
- Receptors, Lysophosphatidic Acid