Chemical alignment of DNA origami to block copolymer patterned arrays of 5 nm gold nanoparticles.

Pearson, Anthony C; Pound, Elisabeth; Woolley, Adam T; Linford, Matthew R; Harb, John N; Davis, Robert C · Nano Lett · 2011

basic_science · Level V

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Abstract

We have used block copolymer patterned arrays of 5 nm gold nanoparticles (AuNPs) for chemically aligned surface attachment of DNA origami. Addition of single-stranded DNA-thiol to AuNPs allowed a base paired attachment of sticky end modified DNA origami. Results indicate a stable, selective attachment between the DNA origami and ssDNA modified AuNPs. Yield data showed 74% of AuNP binding sites forming an attachment with a DNA origami rectangle, and control surfaces showed less than 0.5% nonspecific adsorption.

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